Characterization of an Assay for Kinetic Studies of Acetylcholinesterase.

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Hidayat Ullah
Fazli Rab
Sayed Fazli Mabood

Abstract

The effect different enzyme and substrate preparation on the parameters (akm, aV) of acetyl cholinesterase (AChE)  (EC. 3.1. 1:7) were estimated. Time for preceincubating enzyme with 5,5 dithio – 2 – nityrobenzoic acid (DTNB) were also assessed. The effect of storage on activity of AChE was determined. Moreover the unsatisfactory result of storage of substrate and DTNB on ice during assays were also reported.

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How to Cite
1.
Ullah H, Rab F, Mabood SF. Characterization of an Assay for Kinetic Studies of Acetylcholinesterase. J Postgrad Med Inst [Internet]. 2011 Aug. 25 [cited 2024 Apr. 17];7(2). Available from: https://jpmi.org.pk/index.php/jpmi/article/view/432
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Original Article